In Situ Bio-Reactors

BioEnhance is an in-well treatment technology designed to support and enhance the indigenous microbial activity at sites impacted by petroleum hydrocarbons and chlorinated solvents.

ISBR Design Specifics Include:

  • Designed to fit in a standard 2-in. and 4-in. schedule 40 wells
  • The packed-bed bioreactor contains Bio-Sep beads and is open for fluid flow at the top and bottom.
  • Can be used to enhance either aerobic or anaerobic conditions.
    • Aerobic: Air sparging into the bottom of the packed bed (from a surface compressor) provides oxygen to enhance aerobic biodegradation and creates air lift for circulation of groundwater.
    • Anaerobic: Nitrogen gas from a surface compressed gas source is used to provide gas-lift circulation in the ISBR.
  • Configured for continuous injection of nutrients from a surface source as needed to maintain the growth and reproduction of the microbes.
  • Can be used to transfer adapted indigenous microbial populations from well to well or site to site.
  • Specifically designed to use Bio-Trap samplers within the ISBR and down-gradient to monitor the effects on populations of degraders and maximize performance.
  • BioEnhance is an extension of the Bio-Trap samplers which have been used successfully for a decade to provide converging lines of evidence to evaluate bioremediation as a treatment mechanism and direct site management activities. 
Groundwater flows into the bottom slotted section of the unit, up through the packed bed, then out the top slotted section, transporting microbes into the formation. 

Groundwater flows into the bottom slotted section of the unit, up through the packed bed, then out the top slotted section, transporting microbes into the formation. 

These properties combine to make Bio-Sep an ideal medium for an in situ biotreatment system where indigenous bacteria with biodegradation capabilities may be concentrated for treatment within a groundwater monitoring well and transported away from the wellbore by groundwater flow and growth through the aquifer matrix.